Natural-Immunogenics Corp.
3265 W. McNab Rd, Pompano Beach, Florida
33069
Tel: 954-979-0885, Fax: 954-979-0838
CERTIFICATE
OF ANALYSIS
Date:
October 25, 2004
Client:
John Stewart
Sample
I.D.:
Biophysica
Colloidal Silver (BP)
Date
Manufactured:
None
available
Analysis Requested: Silver Concentration and other standard parameters
Results:
Concentration
& other parameters
Sample
Silver (ppm)
pH
Tyndall
Visual
Conductivity [m
S]
BP
24.20
6.96
++++ Clear
38
Comments:
Concentration Analysis performed in accordance with Perkin-Elmer
Standard Test Procedures.
Seth Nosel
10/25/04
_____________________________________
______________
For
Natural-Immunogenics Corp.
Date
Natural-Immunogenics does not accept any liability concerning the use of these results.
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Natural-Immunogenics Corp. 3265 W. McNab Rd, Pompano Beach, Florida
33069 Tel: 954-979-0885, Fax: 954-979-0838 CERTIFICATE
OF ANALYSIS
Date:
November 1, 2004 Client:
John Stewart Sample
I.D.:
Biophysica
Colloidal Silver (BP) Date
Manufactured:
None
available Analysis Requested:
Particle
Characterization Results:
Sample
- BP
Micrograph
#s 1221 & 1222 (at 100,000x)
Particle Size Min. 1 nm
Max. 8
nm
Comment: Presents as a residue of molecular solution with some colloidal presence as a minor proportion of the silver content. Note the particulate content (max 8 nm; min 1 nm) in the margins of the residue in both micrographs (#1221 and #1222).
Stephen Quinto 11/1/04 _____________________________________
______________ For Natural-Immunogenics Corp. Date Natural-Immunogenics does not accept any liability concerning the use of these results.
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A Comparative Study of the Antibacterial Efficacy of Colloidal Silver (BP) vs. Staphylococcus aureus (colloidal
silver samples submitted by Dr. John Stewart)
October 29, 2004 by Natural-Immunogenics Corp.Purpose. The purpose of this study was to test the inhibition of Biophysica (BP) on both normal and antibiotic resistant strains of Staphylococcus aureus (“Staph”). This was accomplished by innoculating healthy Staph cultures with the colloidal silver on standard YT plates, then placing them in a 37° C incubator overnight Materials
and Methods. Each 95mm sterile polystyrene plate was filled with 5mm of YT media consisting of 0.5% sodium chloride, 0.6% yeast extract, 0.8% tryptone and 2% agar. A small 3 mm scrape of Staph was resuspended in sterile 18 MΩ lab water and diluted. To each 100 µl dilution of staph cells, 10µl of silver was added and then let sit for both 4 minutes and 8 minutes. After the appointed time, five 10µl spots of each diluted staph strain containing silver were arranged on a YT plate. A. The YT media was autoclaved and poured into sterile plates and allowed to dry. B. Two strains of Staph received from the New York Hospital of Queens. 1. B14192 – wildtype/ normal strain -Renamed S-1 2. B14310 – antibiotic resistant (MRSA) -Renamed S-2 C. A small 3 mm scrape of both S-1 and S-2 were resuspended in 1250µl of lab pure water. Then a 10:1 dilution series was performed on each strain. D. Silver was added to each dilution of S-1 and S-2; the cultures were then agitated and allowed to sit until spotted onto the YT plate. The exposure time of each dilution to the silver was both four and eight minutes. E.
Silver
products used: 1. BP
-clear a.
ppm = 24.20 b.
pH = 6.96 c. Tyndall Effect = ++++ (out of a possible ++++) d. Conductivity = 38 [mS] F. Positive control plate -No colloidal silver added to S-1 or S-2 cultures Negative control plate -No Staph or silver added to check for contamination
(image not included, no contamination). G. Plates then placed in a 37°C incubator overnight. Results. Qualitative
results can easily be seen on each plate. The positive control for S-1
grew out 4.5 spots, represented by (++++½) and the positive control for
S-2 grew out 4.5 spots when colloidal silver was not present (++++½). A
(-) represents no Staph grew on the plate. The effectiveness of the
colloidal silver can be compared by the number of spots or +’s as seen
in the graph. Inhibition of Staph @ 4 Minutes S-1
S-2 +
control
++++½ ++++½ BP
++
+½ Inhibition of Staph @ 8 Minutes S-1
S-2 +
control
++++½
++++½ BP
+
+ Inhibition of Staph @ 4 Minutes S-1
S-2 + control
BP
Inhibition of Staph @ 8 Minutes S-1
S-2 +
control
BP
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Disclaimer: The information conveyed here is based on records and research for your information which is for horticultural, educational, sacramental and research purposes only. No statement should be taken as medical advice. It is not claimed or implied here that Mono-atomic SILVER-MAX or Colloidal mineral waters will cure any disease.
Contact: Biophysica Inc., Hamilton (Greater Toronto), Ont, Canada This page last updated on August 5, 2013 |
